The purpose is to study the influence of individual and joint application of humic preparation Lignohumate and microbiological fertilizer Baikal-EM on the structural state and enzymatic activity of ordinary chernozem.
Materials and methods. Soil samples were investigated by the method of dry sieving to calculate the structural coefficient, by the method of wet sieving to determine the number of water-stable aggregates; the analysis of the dynamics of soil humus was carried out; the dynamics of catalase and invertase activity has been studied.
Results. The dynamics of humus during the study period has a similar picture in all variants, however, the end of the growing season in 2011 demonstrated a statistically significant increase in the amount of humus by 0.25 % when a humic preparation and a concentrate of microorganisms are simultaneously applied into soil, in the same variant in the same period, an increase in catalase activity was noted up to 29 ml О₂/(g · min) compared to 20 ml О₂/(g · min) in the control. The application of microorganisms also revealed a correlation between humus and invertase activity: option 4 showed an increase in this indicator to a statistically significant 18 mg glucose/(g · 24 h) in the absence of a significant difference in dynamics in other options. The structural characteristics of soil showed the greatest response to treatment with Lignohumate and Baikal-EM, their application led to the increase in the structure coefficient up to 2.75 compared to 1.6–1.7 in the control variant and with the application of Lignohumate on the leaf. The content of water-stable aggregates in the control variant and with foliar treatment throughout the entire experiment was in the range of 70–75 %, while soil treatment with preparations led to the increase in water resistance of the structure to 90 %.
Conclusions. Chernozem turned out to have response to the Lignohumate, application especially in combination with Baikal-EM, directly into soil, which is associated with the stimulation of its own soil microflora by the applied concentrate of microorganisms.
doi: 10.31774/2712-9357-2022-12-1-177-194
ordinary chernozem, Lignohumate, humic preparation, microbiological fertilizer content of water-resistant aggregates, structural coefficient, catalase, invertase, humus
Dubinina M. N., Lykhman V. A., Bezuglova O. S., Naimi O. I., Polienko E. A. Dynamics of the structural state and enzymatic activity of ordinary carbonate chernozem when using biologically active preparations. Land Reclamation and Hydraulic Engineering. 2022;12(1):177–194. (In Russ.). https://doi.org/10.31774/2712-9357-2022-12-1-177-194.
1. Shivlata L., Satyanarayana T., 2017. Actinobacteria in agricultural and environmental sustainability. Agro-Environmental Sustainability. Cham, Springer, pp. 173-218, https:doi.org/10.1007/978-3-319-49724-2_9.
2. Flores-Félix J.D., Menéndez E., Rivas R., Encarnación Velázquez M., 2019. Chapter 9 – Future perspective in organic farming fertilization: Management and product. Organic Farming. Global Perspectives and Methods. Woodhead Publishing Series in Food Science, Technology and Nutrition, pp. 269-315, https:doi.org/10.1016/B978-0-12-813272-2.00010-0.
3. Wilpiszeski R.L., Aufrecht J.A., Retterer S.T., Sullivan M.B., Graham D.E., Pierce E.M., Zablocki O.D., Palumbo A.V., Elias D.A., 2019. Soil aggregate microbial communities: Towards understanding microbiome interactions at biologically relevant scales. Applied and Environmental Microbiology, vol. 85, no. 14, https:doi.org/10.1128/AEM.00324-19.
4. Blume H.P.K., Brümmer G.W., Fleige H., Horn R., Kandeler E., Kögel-Knabner I., Kretzschmar R., Stahr K., Wilke B.M., 2016. Chemical properties and processes. Scheffer/ Schachtschabel Soil Science. Berlin, Heidelberg, Springer, pp. 123-174, https:doi.org/10.1007/978-3-642-30942-7_5.
5. Rabot E., Wiesmeier M., Schluter S., Vogel H.J., 2018. Soil structure as an indicator of soil functions: A review. Geoderma, vol. 314, pp. 122-137, https:doi.org/10.1016/j.geoderma.2017.11.009.
6. Lamandé M., Schjønning P., 2018. Soil mechanical stresses in high wheel load agricultural field traffic: A case study. Soil Res., 56, pp. 129-135, https:doi.org/10.1071/SR17117.
7. Vezzani F.M., Anderson C., Meenken E., Gillespie R., Peterson M., Beare M.H., 2018. The importance of plants to development and maintenance of soil structure, microbial communities and ecosystem functions. Soil Tillage Res., vol. 175, pp. 139-149, https:doi.org/10.1016/j.still.2017.09.002.
8. Pareja-Sánchez E., Plaza-Bonilla D., Ramos M.C., Larnpurlanes J., Alvaro-Fuentes J., Cantero-Martinez C., 2017. Long-term no-till as a means to maintain soil surface structure in an agroecosystem transformed into irrigation. Soil Tillage Res., vol. 174, pp. 221-230, https:doi.org/10.1016/j.still.2017.07.012.
9. Sahu A., Bhattacharjya S., Mandal A., Thakur J.K., Atoliya N., Sahu N., Manna M.C., Patra A.K., 2018. Microbes: A sustainable approach for enhancing nutrient availability in agricultural soils. Role of Rhizospheric Microbes in Soil. Singapore, Springer, pp. 47-75, https:doi.org/10.1007/978-981-13-0044-8_2.
10. Macias-Benitez S., Garcia-Martinez A.M., Jimenez P.C., Gonzalez J.M., Moral M.T., Rubio J.P., 2020. Rhizospheric organic acids as biostimulants: Monitoring feedbacks on soil microorganisms and biochemical properties. Front. Plant Sci., 11, 633, https:doi.org/10.3389/fpls.2020.00633.
11. Bezuglova O.S., Gorovtsov A.V., Polienko E.A., Zinchenko V.E., Grinko A.V., Lykhman V.A., Dubinina M.N., Demidov A., 2019. Effect of humic preparation on winter wheat productivity and rhizosphere microbial community under herbicide-induced stress. Journal of Soils and Sediments, 19, pp. 2665-2675, https:doi.org/10.1007/s11368-018-02240-z.
12. Lykhman V.A., Klimenko A.I., Dubinina M.N., Naimi O.I., Polienko E.A., 2020. Influence of humic preparations on the content of carbohydrates in structural units and their water resistance. E3S Web Conf. Vol. 210. Innovative Technologies in Science and Education (ITSE-2020), article number: 04005, 12 p., https:doi.org/10.1051/e3sconf/202021004005.
13. Lykhman V.A., Klimenko A.I., Dubinina M.N., Polienko E.A., Naimi O.I., 2020. Vliyanie na agregatnyy sostav chernozema obrabotki posevov bakovoy smes'yu guminovogo preparata i gerbitsida [The influence of treating crops with the tank mixture of the humic preparation and the herbicide on the aggregate composition of chernozem]. Zemledelie [Farming], no. 8, pp. 3-7, DOI: 10.24411/0044-3913-2020-10801. (In Russian).
14. Calvo P., Nelson L., Kloepper J.W., 2014. Agricultural uses of plant biostimulants. Plant and Soil, 383, pp. 3-41, https:doi.org/10.1007/s11104-014-2131-8.
15. Bashan Y., Bashan L.E., Prabhu S.R., Hernandez J.P., 2014. Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013). Plant and Soil, 378, pp. 1-33, https:doi.org/10.1007/s11104-013-1956-x.
16. Vaezi A.R., Eslami S.F., Keesstra S., 2018. Interrill erodibility in relation to aggregate size class in a semi-arid soil under simulated rainfalls. Catena, 167, pp. 385-398, https:doi.org/10.1016/j.catena.2018.05.003.
